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PhD start date: 1. November 2016
Topic: Biosynthetic nucleic acid isotope labeling
In bacteria we can label RNA with the isotopes 15N, 34S and 13C for total labeling and deuterium in methyl groups (CD3). With this isotope labeling in combination with
LC-MS/MS we can identify the sum formula and solve the structure of unknown modifications. We can do enzymatic studies in order to find out if modifications are added to the RNA by accident or enzymatically and we developed strategies to follow modifications over time in pulse chase experiments. For stress initiation we use different compounds, e.g. alkylating agents or antibiotic compounds. Afterwards we look for changed modification patterns in different RNAs and if the RNA was damaged by the stress, we follow the repair and enzyme requisition.