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Stress induced RNA modification mechanisms in yeast

Yeast, stricter S. cerevisiae is among the best-studied model organisms for eukaryotic biology. It encodes similar proteins as mammals, and therefore provides parallels to the kingdom of Animalia, thus to human. Over the last three decades much research has been conducted to examine epigenetics of yeast and other organisms.
Nowadays, there is growing interest in studying the epitranscriptome, specifically in deciphering the types and locations of RNA modifications. Applying our tools for RNA modification analysis we determine the stress response of yeast to certain chemicals. Further, we want to shed light on underlying mechanisms involved in the modification profile adaptions. Besides the determination of the presence of certain modifications, it is of high interest to follow their fate. Usage of different isotope labeling enables us to determine which modification occurred during a certain condition. The used techniques allow us to predict if modified RNA is degraded, fragmented or actively demodified.

Team:

  • Yasi
  • Erik

Publications:

2017

  • Heiss, M., Reichle, V. & Kellner, S. (2017). Observing the fate of tRNA and its modifications by nucleic acid isotope labeling mass spectrometry: NAIL-MS. RNA Biol. 2017 May 10:0. doi: 10.1080/15476286.2017.1325063.