Publications Dr. Ulrich Lächelt

Papers

2024

Germer J, Lessl AL, Pöhmerer J, Grau M, Weidinger E, Höhn M, Yazdi M, Cappelluti MA, Lombardo A, Lächelt U, Wagner E. (2024) Lipo-Xenopeptide Polyplexes for CRISPR/Cas9 Based Gene Editing at Ultra-Low Dose. J. Control. Release 2024 Jun:370:239-255
Berger S, Lächelt U, Wagner E. (2024) Dynamic carriers for therapeutic RNA delivery. Proc Natl Acad Sci U S A. 2024 Mar 12;121(11):e2307799120
Lyu M, Yazdi M, Lin Y, Höhn M, Lächelt U, Wagner E. (2024) Receptor-Targeted Dual pH-Triggered Intracellular Protein Transfer. ACS Biomater Sci Eng. 2024 Jan 8;10(1):99-114
Albuquerque LJC, de Oliveira FA, Christoffolete MA, Nascimento-Sales M, Berger S, Wagner E, Lächelt U, Giacomelli FC. (2024) Nucleic acid delivery to retinal cells using lipopeptides as a potential tool towards ocular gene therapies, J. Colloid Interface Sci. 2024 Feb:655:346-356

2023

Lessl AL, Pöhmerer J, Lin Y, Wilk U, Höhn M, Hörterer E, Wagner E, Lächelt U. (2023) mCherry on Top: A Positive Read-Out Cellular Platform for Screening DMD Exon Skipping Xenopeptide-PMO Conjugates, Bioconjug. Chem. 2023 Dec 20;34(12):2263-2274
Zhou F, Huang L, Li S, Yang W, Chen F, Cai Z, Liu X, Xu W, Lehto VP, Lächelt U, Huang R, Shi Y, Lammers T, Tao W, Xu Z, Wagner E, Xu ZP, Yu H. (2023) From structural design to delivery: mRNA therapeutics for cancer immunotherapy, Exploration (Beijing). 2023 Nov 17;4(2):20210146
Öztürk Ö, Lessl AL, Höhn M, Wuttke S, Nielsen PE, Wagner E, Lächelt U. (2023) Peptide nucleic acid-zirconium coordination nanoparticles, Sci Rep. 2023 Aug 30;13(1):14222
Hall A, Bartek J, Wagner E, Lächelt U, Moghimi SM. (2023) High-resolution bioenergetics correlates the length of continuous protonatable diaminoethane motif of four-armed oligo(ethanamino)amide transfectants to cytotoxicity, J Control Release 2023 Sep:361:115-129
Shen, F., Lin, Y., Höhn, M., Luo, X., Döblinger, M., Wagner, E., Lächelt, U. (2023) Iron-gallic acid peptide nanoparticles as versatile platform for cellular delivery with synergistic ROS enhancement effect, Pharmaceutics 2023 Jun 21;15(7):1789
LinY., Luo, X., Burghardt, T., Dörrer, S., Höhn, M., Wagner, E., Lächelt, U. (2023) Chemical evolution of amphiphilic xenopeptides for potentiated Cas9 ribonucleoprotein delivery, J Am Chem Soc. 2023 Jul 19;145(28):15171-15179
Lin Y, Wilk U, Pöhmerer J, Hörterer E, Höhn M, Luo X, Mai H, Wagner E, Lächelt U. (2023) Folate receptor-mediated delivery of Cas9 RNP for enhanced immune checkpoint disruption in cancer cells, Small. 2023 Jan;19(2):e2205318

2022

Mirzazadeh Dizaji N, Lin Y, Bein T, Wagner E, Wuttke S, Lächelt U, Engelke H (2022) Biomimetic mineralization of iron-fumarate nanoparticles for protective encapsulation and intracellular delivery of proteins, Chem Mater. 2022 Oct 11;34(19):8684-8693
Lyu M, Yazdi M, Lin Y, Höhn M, Lächelt U, Wagner E. (2022) Receptor-Targeted Dual pH-Triggered Intracellular Protein Transfer, ACS Biomater Sci Eng. 2022 Jul 8. doi: 10.1021/acsbiomaterials.2c00476. Online ahead of print
Lin, Y., Wagner, E., Lächelt, U. (2022) Non-viral delivery of the CRISPR/Cas system: DNA versus RNA versus RNP, Biomaterials Science 2022 Mar 2;10(5):1166-1192
Capelôa L, Yazdi M, Zhang H, Chen X, Nie Y, Wagner E, Lächelt U, Barz M. (2022) Cross-linkable Polyion Complex Micelles from Polypept(o)ide-based ABC-triblock Copolymers for siRNA Delivery, Macromol Rapid Commun. 2022 Jun;43(12):e2100698

2021

Krhač Levačić A, Berger S, Müller J, Wegner A, Lächelt U, Dohmen C, Rudolph C, Wagner E. (2021) Dynamic mRNA polyplexes benefit from bioreducible cleavage sites for in vitro and in vivo transfer, J Control Release 2021 Nov 10;339:27-40
Kamenac A, Schilberth FL, Wagner E, Wixforth A, Lächelt U, Westerhausen C. (2021) Transient Permeabilization of Living Cells: Combining Shear Flow and Acoustofluidic Trapping for the Facilitated Uptake of Molecules. Processes. 2021; 9(6):913
Loy, D.M., Krzysztoń, R., Lächelt, U, Rädler, J.O., Wagner, E. (2021) Controlling Nanoparticle Formulation: A Low-Budget Prototype for the Automation of a Microfluidic Platform. Processes 2021, 9(1), 129

2020

Steinborn B., Lächelt U. (2020), Metal-Organic Nanopharmaceuticals, Pharmaceutical Nanotechnoogy 2020, accepted.
Lächelt U., Wuttke S., Engelke H. (2020) Colloidal nanoparticles as pharmaceutical agents, Frontiers of Nanoscience 2020, Volume 16, 89-115. https://doi.org/10.1016/B978-0-08-102828-5.00005-X
Ploetz E., Engelke H., Lächelt U., Wuttke S. (2020) The Chemistry of Reticular Framework Nanoparticles: MOF, ZIF, and COF Materials, Adv. Funct. Mater. 2020. https://doi.org/10.1002/adfm.201909062
Hirschle P., Hirschle C., Böll K., Döblinger D., Höhn M., Tuffnell J.M., Ashling C.W., Keen D.A., Bennett T.D., Rädler J.O., Wagner E., Peller M., Lächelt U., Wuttke S. (2020) Tuning the Morphological Appearance of Iron(III) Fumarate: Impact on Material Characteristics and Biocompatibility, Chem. Mater. 2020, 32, 6, 2253-2263. https://pubs.acs.org/doi/10.1021/acs.chemmater.9b03662
Kuhn J., Lin Y., Krhac Levacic A., Al Danaf N., Peng L., Höhn M., Lamb D.C., Wagner E., Lächelt U. (2020) Delivery of Cas9/sgRNA Ribonucleoprotein Complexes via Hydroxystearyl Oligoamino Amides, Bioconj. Chem. 2020, https://pubs.acs.org/doi/abs/10.1021/acs.bioconjchem.9b00853

2019

Steinborn B., Hirschle P., Höhn M., Bauer T., Barz M., Wuttke S., Wagner E., Lächelt U. (2019) Core-Shell Functionalized Zirconium-Pemetrexed Coordination Nanoparticles as Carriers with a High Drug Content, Adv. Therap. 2019. https://doi.org/10.1002/adtp.201900120
Kuhn J., Klein P.M., Al Danaf N., Nordin J.Z., Reinhard S., Loy D.M., Höhn M., El Andaloussi S., Lamb D.C., Wagner E., Aoki Y., Lehto T., Lächelt U. (2019) Supramolecular Assembly of Aminoethylene-Lipopeptide PMO Conjugates into RNA Splice-Switching Nanomicelles, Adv. Funct. Mater. 2019. https://doi.org/10.1002/adfm.201906432
Holm R., Schwiertz D., Weber B., Schultze J., Kuhn J., Koynov K., Lächelt U., Barz M. (2019) Multifunctional Cationic PeptoStars as siRNA Carrier: Influence of Architecture and Histidine Modification on Knockdown Potential, Macromol Biosci. 2019 Aug 20:e1900152. doi: 10.1002/mabi.201900152.
Zimpel A., Danaf N., Steinborn B., Kuhn J., Höhn M., Bauer T., Hirschle P., Schrimpf W., Engelke H., Wagner E., Barz M., Lamb D.C., Lächelt U., Wuttke S. (2019) Coordinative Binding of Polymers to Metal–Organic Framework Nanoparticles for Control of Interactions at the Biointerface, ACS Nano. 2019 Apr 23;13(4):3884-3895
Schnitzler L.G., Junger S., Loy D.M., Wagner E., Wixforth A., Hörner A., Lächelt U., Westerhausen C. (2019) Size tunable nanoparticle formation employing droplet fusion by acoustic streaming applied to polyplexes, J. Phys. D: Appl. Phys. 2019, 52, 244002. https://doi.org/10.1088/1361-6463/ab131f

2018

Steinborn B., Truebenbach I., Morys S., Lächelt U., Wagner E., Zhang W. (2018) Epidermal growth factor receptor targeted methotrexate and small interfering RNA co-delivery, J Gene Med. 2018 Jul;20(7-8):e3041
Freund R., Lächelt U., Gruber T., Rühle B., Wuttke S. (2018) Multifunctional Efficiency: Extending the Concept of Atom Economy to Functional Nanomaterials, ACS Nano. 2018 Mar 27;12(3):2094-2105

2017

Wang S., Reinhard S., Li C., Qian M., Jiang H., Du Y., Lächelt U., Lu W., Wagner E., Huang R. (2017) Antitumoral Cascade-Targeting Ligand for IL-6 Receptor-Mediated Gene Delivery to Glioma, Mol Ther. 2017 Jul 5;25(7):1556-1566

Krhac Levacic A., Morys S., Kempter S., Lächelt U., Wagner E. (2017) Minicircle Versus Plasmid DNA Delivery by Receptor-Targeted Polyplexes, Hum Gene Ther. 2017 Oct;28(10):862-874

Zhang P, Steinborn B, Lächelt U, Zahler S, Wagner E (2017) Lipo-Oligomer Nanoformulations for Targeted Intracellular Protein Delivery, Biomacromolecules. 2017 Aug 14;18(8):2509-2520

Wang S, Li C, Qian M, Jiang H, Shi W, Chen J, Lächelt U, Wagner E, Lu W, Wang Y, Huang R. (2017) Augmented glioma-targeted theranostics using multifunctional polymer-coated carbon nanodots, Biomaterials. 2017 Oct;141:29-39

Heller P., Hobernik D., Lächelt U., Schinnerer M., Weber B., Schmidt M., Wagner E., Bros M., Barz M. (2017) Combining reactive triblock copolymers with functional cross-linkers: A versatile pathway to disulfide stabilized-polyplex libraries and their application as pDNA vaccines, J Control Release. 2017 Jul 28;258:146-160

Morys S., Krhac Levacic A., Urnauer S., Kempter S., Kern S., Rädler J.O., Spitzweg C., Lächelt U., Wagner E. (2017) Influence of Defined Hydrophilic Blocks within Oligoaminoamide Copolymers: Compaction versus Shielding of pDNA Nanoparticles, Polymers 2017, 9(4), 142

Truebenbach I., Gorges J., Kuhn J., Kern S., Baratti E., Kazmaier U., Wagner E., Lächelt U. (2017) Sequence-Defined Oligoamide Drug Conjugates of Pretubulysin and Methotrexate for Folate Receptor Targeted Cancer Therapy, Macromol Biosci. 2017 Oct;17(10). doi: 10.1002/mabi.201600520

Liu X, Zhang P, Rödl W, Maier K, Lächelt U, Wagner E. (2017) Toward Artificial Immunotoxins: Traceless Reversible Conjugation of RNase A with Receptor Targeting and Endosomal Escape Domains, Mol Pharm. 2017 May 1;14(5):1439-1449

Hall A., Lächelt U., Bartek J., Wagner E., Moghimi S.M. (2017) Polyplex Evolution: Understanding Biology, Optimizing Performance, Mol Ther. 2017 Jul 5;25(7):1476-1490

Beckert L., Kostka L., Kessel E., Krhac Levacic A., Kostkova H., Etrych T., Lächelt U., Wagner E. (2017) Corrigendum to "Acid-labile pHPMA modification of four-arm oligoaminoamide pDNA polyplexes balances shielding and gene transfer activity in vitro and in vivo" [Euro. J. Pharm. Biopharm. 105 (2016) 85-96], Eur J Pharm Biopharm. 2017 Apr;113:240

Röder R., Preiß T., Hirschle P., Steinborn B., Zimpel A., Höhn M., Rädler J.O., Bein T., Wagner E., Wuttke S., Lächelt U. (2017) Multifunctional Nanoparticles by Coordinative Self-Assembly of His-Tagged Units with Metal-Organic Frameworks, J Am Chem Soc. 2017 Feb 15;139(6):2359-2368

2016

Morys S., Wagner E., Lächelt U. (2016) From Artificial Amino Acids to Sequence-defined Targeted Oligoaminoamides, Methods Mol Biol. 2016;1445:235-58

Westerhausen C., Schnitzler L.G., Wendel D., Krzysztoń R., Lächelt U., Wagner E., Rädler J.O., Wixforth A. (2016) Controllable Acoustic Mixing of Fluids in Microchannels for the Fabrication of Therapeutic Nanoparticles, Micromachines 2016; 7, 150; doi:10.3390/mi7090150

Lee D.-J., He D., Kessel E., Padari K., Kempter S., Lächelt U., Rädler J.O., Pooga M., Wagner E. (2016) Tumoral gene silencing by receptor-targeted combinatorial siRNA polyplexes, J Control Release. 2016 Dec 28;244(Pt B):280-291

Zimpel A., Preiß T., Röder R., Engelke H., Ingrisch M., Peller M., Rädler J.O., Wagner E., Bein T., Lächelt U., Wuttke S. (2016) Imparting Functionality to MOF Nanoparticles by External Surface Selective Covalent Attachment of Polymers, Chem. Mater., 2016, 28 (10), pp 3318–3326

Beckert L., Kostka L., Kessel E., Krhac Levacic A., Kostkova H., Etrych T., Lächelt U., Wagner E. (2016) Acid-labile pHPMA modification of four-arm oligoaminoamide pDNA polyplexes balances shielding and gene transfer activity in vitro and in vivo, Eur. J. Pharm. Biopharm. 105, 85–96

He D., Müller K., Krhac Levacic A., Kos P., Lächelt U., Wagner E. (2016) Combinatorial optimization of sequence-defined oligo(ethanamino)amides for folate receptor-targeted pDNA and siRNA delivery. Bioconjugate Chem., 2016, 27 (3):647–659

Liu X., Zhang P., He D., Rödl W., Preiß T., Rädler J., Wagner E., Lächelt U. (2016) pH-reversible cationic RNase A conjugates for enhanced cellular delivery and tumor cell killing. Biomacromolecules, 2016, 17 (1):173–182

Lee D.J., Kessel E., Edinger D., He D., Klein P.M., Voith von Voithenberg L., Lamb D.C., Lächelt U., Lehto T., Wagner E. (2016) Dual antitumoral potency of EG5 siRNA nanoplexes armed with cytotoxic bifunctional glutamyl-methotrexate targeting ligand, Biomaterials. 2016 Jan;77:98-110

2015

Zhang W., Rödl W., He D., Döblinger M., Lächelt U., Wagner E. (2015) Combination of sequence-defined oligoaminoamides with transferrin-polycation conjugates for receptor-targeted gene delivery, J Gene Med. 2015 Aug 9;17(8-9):161-72
Weber J, Lächelt U, Wagner E. (2015) Multifunctional Oligoaminoamides for the Receptor-Specific Delivery of Therapeutic RNA, Methods Mol Biol. 2015;1324:369-86

Broda E, Mickler FM, Lächelt U, Morys S, Wagner E, Bräuchle C. (2015) Assessing potential peptide targeting ligands by quantification of cellular adhesion of model nanoparticles under flow conditions, J Control Release. 2015 Jun 29;213:79-85

Lächelt U., Wagner E. (2015) Nucleic Acid Therapeutics Using Polyplexes – A Journey of 50 Years (and Beyond), Chem Rev. 2015 Oct 14;115(19):11043-78

Kos P., Lächelt U., Herrmann A., Mickler F.M., Döblinger M., He D., Krhač Levačić A., Morys S., Bräuchle C, Wagner E. (2015) Histidine-rich stabilized polyplexes for cMet-directed tumor-targeted gene transfer, Nanoscale. 2015 Mar 12;7(12):5350-62

Kos P., Lächelt U., He D., Nie Y., Gu Z., Wagner E. (2015) Dual-Targeted Polyplexes Based on Sequence-Defined Peptide-PEG-Oligoaminoamides, J Pharm Sci. 2015 Feb;104(2):464-75

2014

Lächelt U, Wittmann V, Müller K, Edinger D, Kos P, Höhn M, Wagner E (2014) Synthetic Polyglutamylation of Dual-Functional MTX Ligands for Enhanced Combined Cytotoxicity of Poly(I:C) Nanoplexes, Mol Pharm. 2014 Aug 4;11(8):2631-9

Zhang CY, Kos P, Müller K, Schrimpf W, Troiber C, Lächelt U, Scholz C, Lamb DC, Wagner E., (2014) Native chemical ligation for conversion of sequence-defined oligomers into targeted pDNA and siRNA carriers. , J Control Release. 2014 Apr 28;180:42-50

Lächelt U., Kos P. Mickler F.M., Herrmann A., Salcher E.E., Rödl W., Badgujar N., Bräuchle C., Wagner E., (2014) Fine-tuning of proton sponges by precise diaminoethanes and histidines in pDNA polyplexes, Nanomedicine. 2014 Jan;10(1):35-44

2012

Dohmen C., Edinger D., Fröhlich T., Schreiner L., Lächelt U., Troiber C., Rädler J., Hadwiger P., Vornlocher H. P., Wagner E., (2012) Nano Sized Multifunctional Polyplexes for Receptor Mediated SIRNA Delivery, ACS Nano. 2012 Jun 26;6(6):5198-208

Martin, I., Dohmen, C., Mas- Moruno, C., Troiber, C., Kos, P., Schaffert, D., Lächelt, U., Teixidó, M., Günther, M., Kessler, H., Giralt, E., Wagner, E., (2012) Solid-phase-assisted synthesis of targeting peptide-PEG-oligo(ethane amino)amides for receptor-mediated gene delivery, Org Biomol Chem. 2012 Apr 28;10(16):3258-68.

Dohmen C., Fröhlich T., Lächelt U., Röhl I., Vornlocher H.-P., Hadwiger P., Wagner E. (2012) Defined Folate-PEG-siRNA Conjugates for Receptor-specific Gene Silencing, Molecular Therapy Nucleic Acids 2012 Jan; 1(1): e7.

2011

Lächelt U., Wagner E. (2011) Invading target cells: multifunctional polymer conjugates as therapeutic nucleic acid carriers, Frontiers Chem. Science Eng. 2011, 5: 275–286.