Kielkowski Research Group - Faculty for Chemistry and Pharmacy

Publications

2022

19. Makarov, D.; Kielkowski, P.: "Chemical proteomics reveals protein tyrosination beyond the alpha-tubulins in human cells." biorxiv, 2022, DOI: 10.1101/2022.07.02.498566.

18. Becker, T.; Wiest, A.; Telek, A.; Bejko, D.; Hoffman-Röder, A.; Kielkowski, P.: "Transforming chemical proteomics enrichment into high throughput method using SP2E workflow." JACS Au, 2022 DOI: 10.1021/jacsau.2c00284.

17. Makarov, D.; Telek, A.; Becker, T.; von Wrisberg, M.; Schneider, S.; Kielkowski, P.: "Clickable report tags for identification of modified peptides by mass spectrometry." J. Mass Spectrom., 2022, 13, e4812. DOI: 10.1002/jms.4812.

2021

16. Becker, T.; Cappel, C.; Di Matteo, F.; Sonsalla, G.; Kaminska, E.; Spada, F.; Cappello, S.; Damme, M.; Kielkowski, P.: "AMPylation profiling during neuronal differentiation reveals extensive variation on lysosomal proteins." iScience, 2021, 24, 103521. DOI: 10.1016/j.isci.2021.103521.

2020

15. Sieber, S. A.; Cappello, S.; Kielkowski, P.: "From young to old: AMPylation hits the brain." Cell Chem. Biol., 2020, 27, 773-779. DOI: 10.1016/j.chembiol.2020.05.009.

TUM (with Prof. Stephan Sieber)

14. Kyrousi, C.; O’Neill, A. C.; Brazovskaja, A.; He, Z.; Kielkowski, P.; Coquand, L.; Giaimo, R. D.; Andrea, P. D.; Belka, A.; Echeverry, A. F.; Mei, D.; Lenge, M.; Cruceanu, C.; Buchsbaum, I. Y.; Khattak, S.; Fabien, G.; Binder, E.; Elmslie, F.; Guerrini, R.; Baffet, A. D.; Sieber, S. A.; Treutlein, B.; Robertson, S. P.; Cappello, S. Extracellular LGALS3BP Regulates Neural Progenitor Position and Relates to Human Cortical Complexity. Nat Commun 2021, 12 , 6298. DOI: 10.1038/s41467-021-26447-w.

13. Rauh, T.; Brameyer, S.; Kielkowski, P.; Jung, K.; Sieber, A. S.: "MS-Based in Situ Proteomics Reveals AMPylation of Host Proteins during Bacterial Infection." ACS Infect. Dis., 2020, 12, 3277–3289.

12. Pfab, M.; Kielkowski, P.; Krafczyk, R.; Volkwein, W.; Sieber, S. A.; Lassak, J.; Jung. K.: "Synthetic post-translational modifications of elongation factor P using (R)-β-lysine ligase EpmA." The FEBS Journal, 2020, 2, 663-677.

11. Buchsbaum, I. Y.; Kielkowski, P.; Giorgio, G.; O’Neill, A. C.; Di Giaimo, R.; Kyrousi, C.; Khattak, S.; Sieber, S. A.; Robertson, S. P.; Cappello, S.: "ECE2 regulates Neurogenesis and Neuronal Migration during Human Cortical Development.", EMBO Rep., 2020, e48204.

10. Pinheiro, B.; Scheidler, C.; Kielkowski, P.; Schmid, M.; Forné, I.; Ye, S.; Reiling, N.; Takano, E.; Imhof, A.; Sieber, S.; Schneider, S.; Jung, K.: "Structure and function of a novel elongation factor P subfamily in Actinobacteria.", Cell Rep., 2020, 30, 4332-4342.e5.

9. Kielkowski, P.; Buchsbaum, I.Y.; Becker, T.; Bach, K.; Cappello, S.; Sieber, S.A.: "A pronucleotide probe for live cell imaging of protein AMPylation", ChemBioChem, 2020, 21, 1285–1287.

8. Kielkowski, P.; Buchsbaum, I.; Kirsch, V.; Bach, N.; Drukker, M.; Cappello, S.; Sieber, S.: "FICD activity and AMPylation remodelling modulate human neurogenesis." Nat. Commun., 2020, 11, 517.

7. Stahl, M.; Korotkov, V. S.; Balogh, D.; Kick, L. M.; Gersch, M.; Pahl, A.; Kielkowski, P.; Richter, K.; Schneider, S.; Sieber, S. A.: "Selective activation of human caseinolytic protease P (ClpP)." Angew. Chem. Int. Ed. 2018, 57, 14602-14607.

PhD (with Prof. Michal Hocek)

6. Cahová, H.; Panattoni, A.; Kielkowski, P.; Fanfrlík, J.; Hocek, M.: "5-Substituted pyrimidine and 7-substituted 7-deazapurine dNTPs as substrates for DNA polymerases in competitive primer extension in the presence of natural dNTPs." ACS Chem. Biol. 2016, 11, 3165-3171.

5. Kielkowski, P.; Cahová, H.; Pohl, R.; Hocek, M.: "Flexible double-headed cytosine-linked 2'-deoxycytidine nucleotides. Synthesis, polymerase incorporation to DNA and interaction with DNA methyltransferases." Bioorg. Med. Chem. 2016, 24, 12768-12776.

4. Kielkowski, P.; Fanfrlík, J.; Hocek, M.: "7-Aryl-7-deazaadenine dNTPs are better substrates for DNA polymerases than dATP in competitive incorporations " Angew. Chem. Int. Ed. 2014, 53, 7552-7555. (Hot paper)

3. Kielkowski, P.; Brock, N. L.; Dickschat, J. S.; Hocek, M.: "Nucleobase Protection Strategy for Gene Cloning and Expression" ChemBioChem 2013, 14, 801-804.

2. Kielkowski, P.; Macíčková-Cahová, H.; Pohl, R.; Hocek, M.: "Transient and Switchable (Triethylsilyl)ethynyl Protection of DNA against Cleavage by Restriction Endonucleases" Angew. Chem. Int. Ed. 2011, 50, 8727–8730.

1. Kielkowski, P.; Pohl, R.; Hocek, M.: " Synthesis of Acetylene Linked Double-Nucleobase Nucleos(t)ide Building Blocks and Polymerase Construction of DNA Containing Cytosines in the Major Groove " J. Org. Chem. 2011, 76, 3457–3462.

Other

Becker, T.; Kielkowski, P.: "Protein-AMPylierungs-Identifikation in lebenden Zellen" BIOspektrum 2020, 07, 743746.